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Exam (elaborations)

TURCAN SBB EXAM REVIEW AABB PACKET {GRADED A+}

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TURCAN SBB EXAM REVIEW AABB PACKET {GRADED A+} Rh inheritence of Rhnull - Lack LW Lack FY5 KEL3 Issues - KPa in cis position may have suppressive effect on other Kell system antigens Adsorption applications in RBC testing - Remove autoantibody Separate multiple antibodies Confir...

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  • May 11, 2024
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  • 2023/2024
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TURCAN SBB EXAM REVIEW AABB

PACKET {GRADED A+}




Rh inheritence of Rhnull - ✔✔Lack LW

Lack FY5

KEL3 Issues - ✔✔KPa

in cis position may have suppressive effect on other Kell system

antigens

Adsorption applications in RBC testing - ✔✔Remove

autoantibody

Separate multiple antibodies

Confirm antigen or antibody specificity

Antilymphocyte Globulin (ALG) - ✔✔Made from horse serum

Interferes with DAT and IAT

Relation to Lu(a-b-)

,PCR and TMA - ✔✔PCR used for DNA amp

TMA used for RNA amp

Autologous donor qualifications - ✔✔DR's order

Hgb >11, HCT 33%

Collected >72 hrs before surgery or Tx

Deferred if risk of bacteremia

Plateletpheresis count info - ✔✔>150,000 min

pre procedure plt count not required for infrequent donors

post-procedure plt count may be used to qualify donor for next

procedure

Two units RBC apheresis post donation H/H - ✔✔Donor HCT not

<30% or Hgb <10 g/dL after volume replacement following

donation

Anticoagulants and purpose of - ✔✔Dextrose:

Supports ATP generation

Adenine:

Provides substrate for ATP synthesis

, Sodium biphosphate:

Controls pH or is buffer

Citrate:

prevents clotting and coagulation

Changes in bag upon storage - ✔✔decreases in:

pH

2,3-DPG

ATP

Increases in:

Hgb

K

Oxygen dissociation curve information - ✔✔p50 is pO2 level at

which Hgb is 50% saturated

A normal p50 is about 25 pO2

increase p50, see right shift, more O2 released

decrease p50, see left shift, less O2 released

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