BSL 1 - ANSdoes not normally cause disease in people
BSL 2 - ANSAssociated with disease
BSL 3 - ANSindigenous/exotic agents that may have serious or lethal consequences and
with potential for aerosol transmission
BSL 4 - ANSdangerous/exotic agents of a life-threatening nature or unknown risk of
transmission
Containment - ANSsafe methods and procedures to handle micro organisms
Aseptic technique prevents contamination of - ANSYou
Others
Experiment
Work Area
External Enviroment
Ubiquitous - ANSfound everywhere
Pathogen - ANSmicro organisms that cause disease
Opportunistic pathogen - ANScause disease in right circumstances
Inoculation - ANSimplantation of micro organisms on or in media
Culture - ANScultivation of the micro organisms
What has actually grown
Turbid - ANScloudy inoculation of growth
Colony - ANSA discrete mass of millions or more cells ideally from one cell
Points of first few labs - ANSpractice aseptic technique
Sterile - ANScompletely free of all life
Pure culture - ANSone type of colony
Mixed culture - ANSmore than one type of colony
, Parfocal - ANSonce its in focus it stays in focus as you move un in magnification
Parcentric - ANSWhat is in the center will stay in the center
Why is oil needed for high magnification - ANSoil is needed to refract light the same as glass
does
4x - ANSscanning
10x - ANSlow power
40x - ANShigh power (high dry)
100x - ANSoil imersion
Total magnification - ANSocular magnification X objective magnification
Working distance - ANSspace between slide and lens
As magnification increases field of view.... - ANSdecreases
Resolution - ANSclarity of an image produced by a set of lenses
Resolving power - ANSability of a lens to separate tow objects in a microscopic field
Function of wavelength of light and a property of the lens called numerical aperture
Numerical aperture - ANSmathematical expression that describes the ability of the
condenser lens to concentrate light rays from the light source
Some refracted light is always lost
What are the four factors for maximum resolution and adequate contrast - ANSBlue filter
Condenser position
Diaphram position
Immersion Oil
What does a three lens system consist of? - ANSOcculars
Objectives
Condenser
Blue filter function - ANSshorter wavelength
Condenser highest position - ANSlimit light lost
Diaphram not completely closed - ANSimproves contrast but reduces NA
immersion oil - ANSforms continuous lens system
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