BIO 171 Lab Exam 1 to 8
1. What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which
would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your
answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will
take several hours to achieve the same effect.
4. What type of incubator is pictured below?
,Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and others from
contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Objective, Procedure, Notes, Results and Deviations
Answer the following questions.
1. You are a lab instructor and Paul Smith has turned in his lab notebook for you to grade the lab
experiment #2 on microscopy. Based upon what was covered in the lab video, how should Paul
have entitled his lab experiment?
PS02 Microscopy
2. You arrive to your first day of work at a new lab. You are taking over for someone who took a new job at
another lab. Your boss informs you that because of time restraints, this person did not exactly follow the
experimental protocol. In order to proceed, you must know what he did differently. (1) According to the lab
lecture, under what section of his notebook would you look to find the experimental steps? (2) As changes
to the experimental steps were made, what are these differences called and how should it appear in the lab
notebook?
, (1) Procedure—this is where the steps for the experimental protocol are recorded.
(2) Deviations. All deviations should be written in red to immediately bring attention to the changes in the protocol.
Answer the following questions
1. Identify the part of the microscope indicated by the arrow.
Neck (or arm)
2. Identify the part of the microscope indicated by the arrow.
Stage
3. Identify the part of the microscope indicated by the arrow.
fine focus
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