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MIBO 3500 EXAM 1 WITH 100% CORRECT ANSWERS

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MIBO 3500 EXAM 1 WITH 100% CORRECT ANSWERS

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  • November 14, 2023
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  • 2023/2024
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MIBO 3500 EXAM 1 WITH 100%
CORRECT ANSWERS
microbe - answer living single-celled organism that requires a microscope and has the capacity
to reproduce


*some microbes can be seen without a microscope*


naming system used:________;


1st word: _________________; always ____________;
2nd word: _______________;


full name is always: ____________ - answer binomial;


genus --> capitalized;
specific epithet;


italicized or underlined


Who built the compound microscope and coined the term "cell"? - answer Robert Hooke


Who first observed single-celled microbes? - answer Antonie van Leeuwenhoek


spontaneous generation - answer living creatures could arise without parents


*rejected by many scientists

,Who demonstrated that microbes cause fermentation and food spoilage and is known as the
father of MIBO? - answer Louis Pasteur


importance of swan-neck flasks - answer determined that diseases have identifiable causes


Who configured the 3 domains? (eukarya, archaea, and bacteria) - answer Carl Woese


Magnification of light microscope - answer 1000x


what happens in a light microscope? (bright-field microscope) - answer light passes thru
specimen --> then thru a series of magnifying lenses


magnification of electron microscope - answer 100,000x


magnification of scanning probe microscope - answer view at atomic level


wavelength of visible light - answer 400 - 750 nm


absorption - answer photon's energy is acquired by the absorbing object


reflection - answer a wavefront bounces off the surface of an object


refraction - answer bending of light as it enters a substance that slows its speed


scattering - answer a wavefront interacts with objects smaller than the wavelength of light


the 3 elements of light microscopy - answer magnification, resolution, contrast

, magnification - answer the ratio of the size of an object to the size of its image --> image is seen
as larger than object


-refracts light to spread its waves (bends the light precisely)


components of a compound microscope - answer 2 magnifying lenses:
ocular lens (10x) and objective lens (greater than 100x)


total magnification is product of ocular and objective lenses


resolution - answer ability to resolve two objects very close together;


quantified as the minimum distance between two objects where they still appear separate


what does resolution depend on? - answer quality of lens and wavelength of light


empty magnification - answer magnification without increasing detail --> results in highly
pixellated image


what is the detail of an image limited by? - answer the wave nature of light --> light rays
undergo interference


higher quality lens = better resolution = ____________ - answer higher Numerical Aperture


name a method that increases resolution - answer immersion oil method - decreases bending of
light since n(oil) is approximately equal to n(glass)

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