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UNE BIOCHEMISTRY UNIT 2 TEST QUESTIONS WITH COMPLETE SOLUTIONS.
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UNE BIOCHEMISTRY UNIT 2 TEST QUESTIONS WITH COMPLETE SOLUTIONS.
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UNE BIOCHEMISTRY UNIT 2 TESTQUESTIONS WITH COMPLETE
SOLUTIONS
3, 2, 4, 1 - answer Order the following statements to illustrate the correct cascade of events that
occurs after ligand binding of G-protein coupled receptor (heptahelical receptor).
1. Degradation of cAMP by phosphodiesterase
2. Dissociation of Gβ and Gγ subunits
3. G protein exchanges GDP for GTP in the G-αs subunit
4. Activation of adenylyl cyclase (effector E) to generate cAMP
Nuclear localization signal - answer A suspected mutation in the cortisol receptor is discovered.
Cortisol can still bind its receptor but the dimerized complex remains in the cytosol. Based on
this description which region on the receptor is likely impacted?
Free fatty acids - answer What is the primary oxidized fuel for skeletal muscle in the fasted
state?
Glucagon - answer Caffeine is a natural inhibitor of phosphodiesterase. If you were to consume
a large amount of coffee you would likely see a prolonged response to which of the following
stimulants or hormones?
Protein Kinase B - answer In the event that PI-3 kinase is not activated downstream of insulin,
which of the following proteins would not be able to dock to the membrane?
, Gluconeogenesis - answer A patient is found to have a rare disease in which the secretory
function of the α-cells of the pancreas is impaired. As a consequence, glucagon cannot be
secreted. Direct stimulation of which of the following pathways in liver will be impaired?
Phosphodiesterase - answer What enzymes is responsible for the degradation of cAMP?
VLDL particles - answer Insulin stimulates fatty acid synthesis in the liver. These fatty acids are
transported to the adipose tissue as which of the following?
Phosphofructokinase-1 (PFK1) - answer After a meal, fructose 2,6-bisphosphate levels are
elevated. This compound will allosterically activate which of the following glycolytic enzymes?
Aconitase - answer A farmer finds a cow dead in his field and notices an unusual plant nearby.
Autopsy results show an elevated level of citrate present in the cow's mitochondria. This data
collectively suggest inhibition of which of the following enzymes?
Pyruvate - answer A deficiency in thiamine can reduce the activity of the pyruvate
dehydrogenase complex. Under these conditions, you would expect to have a higher than
normal blood concentration of which of the following metabolites?
Succinate thiokinase - answer A 10-year-old girl is swimming in a local competition. The intense
exercise will increase intracellular calcium levels which in turn will increase flux through the TCA
cycle by stimulating the activity of all of the following enzymes EXCEPT?
Phosphorylation - answer In order for glucose to be retained within the cell, which of the
following modifications must occur?
Thiamine diphosphate → lipoamide → FAD - answer Which of the following outlines the order
of prosthetic groups as they are used for catalysis within the pyruvate dehydrogenase complex
(PDC)?
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