Exam (elaborations)
Potentail questions of SMT ( with answers )
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All those questions are made by me and they are based on my knowledge of this subject. Those questions are NOT taken form the exam.
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Potential quesitions of SMT,
, MCQ. Easy (40 Questions)
Q1. What is the name of the Ministerial Regulation on GMOs?
A. The Dangerous Substance Act
B. The Environmental Management Act
C. The GMO Decree
D. The ML I document
Q2. What should be done to prevent contamination between experiments?
A. Wear gloves
B. Avoid touching the face and hair
C. Prevent the spread of microorganisms
D. Take appropriate measures
Q3. What is the first step in a virus infection?
A. Inside the cell, virus is uncoated, DNA/RNA is replicated by the host cell
B. The entry of a microorganism into the body of a living being, the host,
and the replication of the microorganism in the host
C. Virus bind to cell (receptors on surface host cell) and penetrates it
D. New virus particles form from these proteins, they get released and cell
dies
Q4. What must be checked as soon as possible on receipt of the material?
A. Identity and purity
B. Disinfectants
C. Waste containers
, D. Permits
Q5. According to the content, which of the following is advised against while
working in the laboratory?
A. Eating
B. Drinking
C. Smoking
D. Wearing cosmetics
Q6. What should be done if the lab coat becomes contaminated?
A. Replace the lab coat immediately
B. Put the lab coat in a sealable plastic bag
C. Decontaminate the lab coat
D. Wash the lab coat
Q7. What is the requirement for working with GMOs?
A. A permit has been obtained under the environmental management act
B. Guidelines and provisions in the GMO decree
C. Notification in advance to the national institute for public health and
environment (RIVM)
D. All of the above
Q8. According to the content, what must be placed in a container containing
liquid nitrogen?
A. Genetically modified microorganisms
B. Non GMO's
, C. GMOs
D. Vectors
Q9. What measures should be taken to protect the experiment and to ensure that
undesirable organisms are not brought into culture?
A. Store the material safely
B. Check the identity of the material each time
C. Take a sample to check whether the material has become contaminated
during culture
D. Pack the material in hermetically sealed, unbreakable, leak proof double
container
Q10. According to the content, when should the purity and identity of the material
be checked?
A. Every three months
B. After the strain or cell line is characterized
C. Every time the fresh stock is needed
D. Before beginning the experiment
Q11. What must be displayed in case of a power failure in the storage place?
A. GMO's
B. Telephone number of the designated researcher (DR/VM) / research
leader (RL/OL)
C. Number of the Environmental Management Act
D. Date
,Q12. What should be done if fluids are to be collected in the waste container?
A. Place them in a sealable container
B. Absorb them into a solid substance
C. Decontaminate them
D. Inspect them
Q13. What is the first layer of protection against microorganisms?
A. Disinfectant
B. Autoclaving
C. Sterilization
D. Tube or bottle that contains the microorganism
Q14. Which of the following is not a biological agent?
A. Bacteria
B. Cell Lines
C. Viruses
D. Fungi
Q15. What is forbidden by law to work with?
A. Plant pathogens
B. Microorganisms in class 2
C. Animal pathogens
D. GMOs
Q16. What must be noted in the lab journal under the relevant date?
, A. Origin of the material
B. Results of the checks
C. Nature of the genetic modification
D. All of the above
Q17. What must be done in the case of a serious ML I incident?
A. Notify the BSO
B. Cover any GMO spill using enough tissues, and impregnate with proper
disinfectants
C. Warn other persons present
D. Disinfect and clear up immediately
Q18. What is the outer layer of protection against microorganisms?
A. laboratory biological containment
B. Disabling the microorganisms
C. Shelf life
D. Training and discipline of the lab worker
Q19. According to the content, what must be placed in the central storage
facility?
A. Vectors, Plasmids, Constructs, Hosts, GMOs, Viruses, Cell lines
B. Constructs, Hosts, GMOs, Viruses
C. Constructs, Hosts, GMOs
D. Vectors, Plasmids, Constructs
,Q20. What is the first priority if there are injuries in the event of a minor ML I
incident?
A. Notify the BSO
B. Disinfect and clean up
C. Warn other persons present
D. First Aid
Q21. How often must the work surface be disinfected after use?
A. Once a week
B. Once a month
C. Immediately after a spillage
D. At least once a day
Q22. What should be placed in a locked location outside work areas?
A. Non GMO's
B. Genetically modified microorganisms
C. GMOs
D. Constructs
Q23. What must be done to the container with contaminated waste before
transport?
A. Open it
B. Store it in the laboratory area
C. Decontaminate it on the outside
, D. Label it
Q24. What is the smallest infectious particle?
A. Fungi
B. Bacteria
C. Viruses
D. Yeast
Q25. What must be marked in the outer packaging of GMOs?
A. GMO notification number
B. Surname or initials of the person who is working with the GMO
C. Date
D. All of the above
Q26. What is dual use research code of conduct made up by KNAW?
A. Guidelines on how to proceed with this type of research to prevent
misuse
B. A law forbidding the intentional misuse of biological agents
(microorganisms)
C. A register listing staff who work with microorganisms in classes 3 and 4
D. A permit issued for the work involving GMOs carried out in the
laboratory
Q27. What should be done with materials obtained commercially (from industry
or a recognized culture collection)?
A. Divide into portions and store safely
, B. Freeze dried material
C. Take a sample to check whether the material has become contaminated
during culture
D. Pack the material in hermetically sealed, unbreakable, leak proof double
container
Q28. What is the classification of animal byproducts?
A. 1,2,3,4,5
B. 1,2,3,4
C. 1,2,3
D. 1,2
Q29. What must be done if activities involving non GMOs are carried out in the
ML I room?
A. Seek permission from the BSO
B. Consult the DR, RL or BSO
C. Apply the ML I work instructions
D. Obtain permission from the Radiation Protection Unit
Q30. According to the content, what must be done before transport of GMO's
outside the laboratory?
A. Obtain permission from the BSO
B. Place the sealed holder in a second container
C. Decontaminate the holder
D. Inspect the holder
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