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Collin College BIOL 1408 Unit 9 Cellular Respiration Packet
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- BIOL 1408
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- Collin College
Collin College BIOL 1408 Unit 9 Cellular Respiration Packet
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Unit 9Cellular Respiration
Abstract
In the macromolecule lab, you learned about carbohydrates, lipids, and proteins and
their importance in biological systems. Furthermore, in the enzyme lab you learned
about the ability of lactase to facilitate the metabolism of lactose, resulting in the
production of galactose and glucose. In this week’s lab we will discuss the importance
of the process known as respiration. In this process, oxygen and glucose are converted
into carbon dioxide, water, and energy in the form of adenosine triphosphate (ATP).
ATP is a molecule that is utilized by many proteins and enzymes in both prokaryotic
and eukaryotic cells in order to do cellular work. In eukaryotes, respiration is primarily
carried out in a cellular organelle known as the mitochondria. In the presence of
oxygen, the respiration that is carried out is known as aerobic, while anaerobic
respiration is carried out in the absence of oxygen.
C6H12O6 + 6O2 à 6CO2 + 6H2O + Energy (ATP)
9.1 O2 Consumption as a Measure of Metabolic Rate
Introduction
Respiration is part of an elaborate metabolic process that enables organisms to do work in
order to maintain homeostasis. This process requires the metabolism of a carbon skeleton
such as glucose as the source of energy and the consumption of oxygen as the final
electron acceptor. In this experiment we are going to measure oxygen consumption to
quantitatively measure the metabolic rate. The organism to be used for this experiment is
a rat. Remember that this particular experiment yields a QUANTITATIVE result, and
therefore, you will have numeric values for your data table.
Animal Average Mass (g) ml O2/h/g
Hamster 80-120 2.3
Dog 3200-54,000 0.32
Man 67500-88600 0.22
Elephant 2050000-6000000 0.07
Figure
1.
Quantitative
differences
in
respiration
between
organisms.
120
, Use the information given in Figure 1 to write a hypothesis about the amount of oxygen
you will observe the rat consuming (measured in ml O2/h/g) compared to other
organisms.
Hypothesis
Based on what I know about the oxygen consumption of different
organisms in figure 1, the rat will _________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________.
Materials and Methods
Note
This particular experiment utilizes a computer program called Physio Ex to
®
simulate an actual experiment that scientists perform. Please follow the
directions below to carry out the experiment. Answer the accompanying
questions to verify that you understand what is going on. DO NOT just race
through the instructions as questions pertaining to this experiment may
appear on your practical.
1. Click on the “Physio Ex 8.0 ” icon on the computer’s desktop.
®
2. On the Physio Ex homepage you will see a drop down menu at the top center,
®
click on the drop down menu and select “Exercise 4: Endocrine System” and
click the “Go” button beside it.
3. The next page should have a series of numbers, with accompanying experiments.
Under #3, click the link labeled “Metabolism.”
4. The following window will appear in your browser:
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