Portage Learning: Microbiology Module 3 Latest 2022 Define the measurements micrometer and nanometer. A micrometer (μm) is defined as being one-millionth of a meter and is commonly designated at 10-6 meters. A nanometer (nm) equals 10-9 m or one-billionth of a meter.
What are the two critical fac...
Portage Learning: Microbiology Modu le 3 Lates t 2022 Define the measurements micrometer and nanometer. ✔✔A micrometer ( µm) is defined as being one-millionth of a meter and is commonly designated at 10 -6 meters. A nanometer (nm) equals 10-9 m or one -billionth of a meter. What are the two critical factors th at influence your ability to see an object? ✔✔Resolution and contrast. Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low resolution means two (or more) objects may appear as one. The contr ast is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) vers us the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark objects = high contrast). If you wish to increase the amount of light going into a microscope, what part would you adjust? ✔✔The iris diaphragm controls the amount of li ght that passes through the sample and into the objective lens. Thus, as you open the iris more light is permitted to pass through to illuminate the sample. As light passes through a microscope, what is the last piece that light passes before reaching you r eyes? ✔✔Once light passes through the sample and the objective lens it is directed through the ocular lens, or eyepiece, directly into your eye . How is the total magnification of an object calculated? ✔✔Total magnification is calculated by multiplying t he power of the objective and the power of the eyepiece. For instance, a 40x objective with a 10x eyepiece would make an object appear (40 x 10) 400 times larger (400x).
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