What would happen in terms of the colorimetric readout if you forgot to do
the wash step after Step D (above)?
Your Answer:
of the wells would have a dark color. because the intensity of the readout
is dependent upon the amount of the labeled
secondary antibody present in the well
All of the wells would have a dark color. The intensity of the
readout is dependent upon the amount of the labeled
secondary antibody present in the well. Since the unbound
secondary antibody was not washed away, all wells would
react equally.
Question 4 2..5 pts
A western blot separates proteins based on ______________.
Your Answer:
Its size
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