1. Which germicide was the most effective? Was the same germicide most effective for bacteria
isolated from the different sources? What could explain this finding?
10% bleach was most effective. Yes, it was most effective for bacteria isolated from the different sources.
Since bleach is a soluti...
straighterline bio250l lab 7 microbial control 2022
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Lab 7 Microbial Control BIO250L
Student Name:
Kit Code (located on the lid of your lab kit):
Experiment 1- Effect of Chemical Germicides on Bacterial Growth
Table 3: Experiment Results
Sample Germicide Zone of Inhibition (mm) Relative Effectiveness
10% Bleach 15-16 Yes
70% Isopropyl
7-6 Minimal
Skin Alcohol
Hibiclens™ 11-15 Yes
Control 0-2 No
10% Bleach 10-11 Yes
70% Isopropyl
6-7 Minimal
Nose Alcohol
Hibiclens™ 8-12 Yes
Control 0-1 No
10% Bleach 12-18 Yes
70% Isopropyl
6-7 No
Throat Alcohol
Hibiclens™ 10-12 Yes
Control 2-4 No
10% Bleach 12-14 Yes
70% Isopropyl
6-7 No
Shoe Alcohol
Hibiclens™ 10-12 Yes
Control 3-2 No
Post-Lab Questions
1. Which germicide was the most effective? Was the same germicide most effective for bacteria
isolated from the different sources? What could explain this finding?
10% bleach was most effective. Yes, it was most effective for bacteria isolated from the different sources.
Since bleach is a solution of sodium hypochlorite and water that is very effective in killing
microorganisms, it is capable of entering microbial cells where it can react with many cellular
components, disrupting and killing them.
2. Which germicide was the least effective? Was the same germicide least effective for bacteria isolated
from different sources? What could explain this finding?
70% Isopropyl alcohol was least effective. Yes, it was least effective on the bacteria isolated frlm different
places. This could be because, it erodes quicklg if left uncapped, evaporating most of its alcohol leaving
behind only water which is not strong enough to destroy bacteria.
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