Summary
Chapter 7 summary (college+book) (Molecular Biology of the Cell)
- Course
- Institution
- Book
Summary of chapter 7 from Alberts. Summary consists of lecture notes and information from the book including pictures.
[Show more]
Connected book
Book Title:
Author(s):
- Edition:
- ISBN:
- Edition:
More summaries for
-
Unlock Success with the [Molecular Biology of the Cell,Alberts,6e] 2023-2024 Test Bank
-
Hoofdstuk 9 samenvatting (college+boek)(Molecular Biology of the Cell)
-
Hoofdstuk 6 samenvatting (college+boek)(Molecular Biology of the Cell)
Seller
Follow
julia39
Content preview
Hoorcollege 7-> each protein domain recognizes a relatively short sequence, but duplication or tandem
multimerization results in longer sequence targets
helix-turn-helix proteins -> eiwit komt ertussen in de grote groeve van het DNA
- proteins can “read” DNA sequence via interaction in major groove
- helix-turn-helix protein
- homeodomain protein
- leucine zipper protein
- helix-loop-helix protein
- b sheet DNA recognition proteins
- zinc finger proteins
transcription factors can bind to DNA cooperatively
- when they bind together to the DNA, the affinity is higher
- when they bind separately to the DNA, the affinity is lower
nucleosomes can promote cooperative binding of transcription factors to DNA
conclusions:
- eukaryotic gene transcription is regulated on a per gene basis
- transcription factors recognize specific sequences and act cooperatively to regulate
transcription
~5-10% of all genes code for transcriptional regulators
RNA seq -> identify exons and illustrate differential gene expression in different cell types
regulatory DNA defines gene expression patterns during development and cell differentiation
- the genome is the same in all cell types, but different genes are active in different
tissues because different transcription factors bind to them
, ChIP assay: chromatin immunoprecipitation
- to which sequence within the genome does a TF bind?
- which TF binds to a given regulatory element?
finding regulatory sequences -> use of reporter genes
- stuk DNA pakken uit genoom en plakken achter een gen dat makkelijk zichtbaar is
- zodra deze wordt afgeschreven en zichtbaar is in de cel kun je ook direct zien wat
dat stuk DNA doet (like LacZ or GFP)
discovering gene expression patterns ->use of RNA in situ hybridization
- look at expression of mRNA in certain tissues
-> regulatory elements controlling the expression of TF and developmental regulators
diverged in early vertebrate evolution
-> cis-regulatory elements of an eukaryotic genen can be spread over long distances and
come together through DNA looping
the large size of gene control regions complicates molecular definition of a ”gene”
most eukaryotic genes are controlled independently of each other
-> eukaryotic transcription regulators bind weakly to each other and assemble into
complexes on the DNA
heel veel regulatoren kunnen als enhancers én als repressors dienen
-> transcription activators alter chromatin structure before DNA transcription/replication
histone acetyltransferase histone kinase and chromatin remodeling complex are
transcription co-activators
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller julia39. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.81. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75323 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now