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Measuring Gene Expression: Theory, Techniques and Application
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Lecture notes on Measuring Gene Expression: Theory, Techniques and Application
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https://www.youtube.com/watch?v=1kvy17ugI4whttps://www.youtube.com/watch?v=0ATUjAxNf6U https://www.youtube.com/watch?v=G2Z1wuEOZUU
https://www.promega.co.uk/resources/multimedia/reporter-assays-and-transfection/introduction-to-reporter-gene-
assays/
Measuring Gene Expression: Theory, Techniques and Application
Regulation of Gene Expression: Transcription
The Fireflies:
, - Order Coleoptera: Family Lampyridae
- 2,000 described species
- Produce bioluminescent light from the lower abdomen during twilight to attract mates or prey
- Light may be yellow, green, or pale red, with wavelengths from 510 to 670 nm
The Chemistry of Bioluminescence:
Reporter Gene Assays: Principle II
Reporter Gene Assays: Applications
- Understanding promoter structure
- Analysis of transcription factor structure
- Activation of cell receptors
- Studying effects of drugs/treatments on gene expression
Types of eukaryotic RNA:
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