LSH4801 PORTFIOLIO (QUESTIONS & ANSWERS) 2024 - DUE 22 November 2024 ;100 % TRUSTED workings, Expert Solved, Explanations and Solutions. For assistance call or W.h.a.t.s.a.p.p us on ...(.+.2.5.4.7.7.9.5.4.0.1.3.2)...........
QUESTION 1 [30]
1.1. Clinical laboratories routinely process specimens ...
LSH4801 PORTFIOLIO (COMPLETE ANSWERS) 2024 - DUE 22 November 2024; 100% TRUSTED Complete, trusted solutions and explanations. Ensure your success with us...
LSH4801 PORTFIOLIO (COMPLETE ANSWERS) 2024 - DUE 22 November 2024; 100% TRUSTED Complete, trusted solutions and explanations. Ensure your success with us...
LSH4801 PORTFIOLIO (COMPLETE ANSWERS) 2024 - DUE 22 November 2024; 100% TRUSTED Complete, trusted solutions and explanations. Ensure your success with us...
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Advanced Medical Microbiology (LSH4801)
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LSH4801
PORTFOLIO 2024
UNIQUE NO.
DUE DATE: 22 NOVEMBER 2024
, LSH4801
Portfolio 2024
Unique No.
Due Date: 22 November 2024
Advanced Medical Microbiology
Question 1
1.1 Immunofluorescence to Identify Streptococcus pyogenes
Application of Immunofluorescence:
Immunofluorescence is a diagnostic method that uses fluorescent-labeled antibodies to
detect specific antigens on microorganisms. To identify Streptococcus pyogenes:
Antibody Preparation: Use fluorescent-labeled monoclonal antibodies
specifically designed to bind to antigens unique to S. pyogenes (e.g., the M
protein or group A carbohydrate antigen).
Sample Preparation: Smear the isolated patient sample onto a glass slide and
fix the bacterial cells with heat or chemicals.
Antibody Application: Add the fluorescent-labeled antibodies to the slide. After
incubation, unbound antibodies are washed off.
Microscopic Examination: Use a fluorescence microscope to observe specific
binding. A positive identification of S. pyogenes will show fluorescence on the
bacterial cells.
Differentiation from Morphologically Similar Organisms:
1. Distinction from Other Streptococci:
o S. pyogenes belongs to Lancefield Group A. Perform a Lancefield
grouping test (serological grouping) to confirm the presence of group A
carbohydrate antigen.
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