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BIO 219 QUIZZES Questions With Complete Solutions

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BIO 219 QUIZZES Questions With Complete Solutions Which gene in the lux operon encodes the auto-inducer, AHL Lux R b. Lux I c. Lux A d. Lux D b What is the overall purpose of the quarter-long project? I. To map the lux operon II. To isolate the lux operon from A. fischeri and transf...

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  • November 12, 2024
  • 34
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BIO 219
  • BIO 219
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BIO 219 QUIZZES Questions With Complete
Solutions

Which gene in the lux operon encodes the auto-inducer, AHL

Lux R

b.

Lux I

c.

Lux A

d.


Lux D b




What is the overall purpose of the quarter-long project?

I. To map the lux operon

II. To isolate the lux operon from A. fischeri and transform E. coli with it the operon to induce

bioluminescence


III. To induce the upregulation of the gadA gene in E. coli through quorum sensing 2 and

3

,BIO 219 QUIZZES Questions With Complete
Solutions
Regulation of gene expression in response to changes in cell population density is known as

_______________________. quorum sensing




If you wanted to get a brighter bioluminescence from your culture of Allivibrio which of the

following "treatments" would increase the amount of blue-green light emitted?




treatment with CO2

b.

Treatment with chaotropic salts

c.

treatment with O2

d.

treatment with acid

e.


Treatment with a alkaline buffer c




What is used to elute the DNA from the silica membrane in the spin column?

a.

,BIO 219 QUIZZES Questions With Complete
Solutions
Chaotropic salts

b.

SDS (sodium dodecy sulfate)

c.

Water

d.

Ethanol

e.


A low pH buffer. c




Good minions always wear their [x] and [y] for lab safety! gloves


goggles




What is the range for the 260:280 ratio that indicates a DNA sample of "good purity"?

2.2 - 3.5

b.

0.5 - 1.5

c.

, BIO 219 QUIZZES Questions With Complete
Solutions
2.0 - 2.2

d.

1.5 - 1.8

e.


1.8 - 2.0 e




Of the assignments listed in the answers, which one is NOT done as group work?

Final Exam

b.

Lab Report 1

c.

Lab Notebooks

d.


Lab Report 2 final exam




A 260:280 ratio = 2.36 would indicate protein contamination. false

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