Exam (elaborations)
Biochemistry 2L06 – Inquiry in Biochemical Techniques Term 1 Lab 4 ELN McMaster University
- Course
- Institution
Biochemistry 2L06 – Inquiry in Biochemical Techniques Term 1 Lab 4 ELN McMaster University
[Show more]
Content preview
Biochemistry 2L06 – Inquiry in Biochemical TechniquesTerm 1 Lab 4 ELN McMaster University
Term 1 Lab 4 ELN
In preparation for the lab day, please read the lab in its entirety and fill out the following sections. Thank
you. This ELN is due Wednesday October 30 th no later than 11 am in the appropriate assignment folder
on A2L. Please submit one pdf file of this completed template. Please note: it is your responsibility to
ensure that your submitted files are not corrupt and can be properly opened or your submission will be
incomplete. Additionally, you must submit in the appropriate assignment folder on A2L otherwise your
submission will be incomplete.
Purpose and experimental overview
In the space provided below:
1. Purpose - Briefly describe the purpose of the lab. What is your overall goal for this lab day
(very concise, cannot exceed 200 words)?
The purpose of this lab is to isolate the E.coli DH5 cells and expose them to alkaline lysis in order
to obtain the plasmid DNA containing the folA gene. The overall goal for this lab is to gain
experience in reading, understanding, and extracting vital information from a kit manual.
2. Experimental overview – Please create a flowchart (using software such as PowerPoint) to highlight
the miniprep procedure (please include diagrams, safety considerations, brief protocol, incubation
times, centrifugation speeds, etc.). The flowchart must be your original work and cannot be copied
directly from the PureLink manual (please note: you will be purifying your plasmid DNA using a
centrifuge). Please also include the cell growth (page 80) and lysis procedure prior to the actual
DNA purification procedure. Also, page 83 of your courseware has some specific requirements for
the miniprep procedure. Please include this information in your flowchart.
, Marking Scheme (for lab mentor use only) – for the purpose and experimental overview section, please provide a mark
out
of 4, whereby:
0-0.5 0.5-1 1-1.5 1.5-2
Purpose - Is concise, uses proper grammar/scientific
Unacceptable Sufficient Accomplished Exemplary
terminology, and appropriately describes the day’s lab. 🗆 🗆 🗆 🗆
Experimental overview – is visually appealing, easy to follow 0-0.5 0.5-1 1-1.5 1.5-2
and understand, contains appropriate content. Shows a clear Unacceptable Sufficient Accomplished Exemplary
understanding of the experimental procedure. 🗆 🗆 🗆 🗆
Mark (/4) and comments:
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller smartzone. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
79223 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now
