Exam (elaborations)
Biochemistry 2L06 – Inquiry in Biochemical Techniques Term 1 Lab 1 ELN McMaster University
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Biochemistry 2L06 – Inquiry in Biochemical Techniques Term 1 Lab 1 ELN McMaster University
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Biochemistry 2L06 – Inquiry in Biochemical TechniquesTerm 1 Lab 1 ELN McMaster University
Term 1 Lab 1 ELN
In preparation for the lab day, please read the lab in its entirety and fill out the following sections. Thank
you. This ELN is due Wednesday September 25 th no later than 11 am in the appropriate assignment
folder on A2L. Please submit one pdf file of this completed template. Please note: it is your responsibility
to ensure that your submitted files are not corrupt and can be properly opened or your submission will
be incomplete. Additionally, you must submit in the appropriate assignment folder on A2L otherwise
your submission will be incomplete.
Purpose and experimental overview
In the space provided below:
1. Purpose - Briefly describe the purpose of each part of this lab. What is your overall goal for this
lab day (very concise, cannot exceed 100 words)?
The Laboratory Basics – Pipetting, & Preparing Solutions section achieves many objectives. Students
are able to gain practical experience in calculating concentrations and amounts, in pipetting
accurately and precisely, and lastly gain an understanding of solution making. In the second portion
of this lab, students will be able to understand the processes involved in PCR through practical
experience.
2. Experimental overview – Design a concise flowchart to highlight the MAIN steps of your
experimental lab setup (not too much detail please and do NOT copy the procedure from
your courseware). Describe the general flow of experiments that will be conducted to address
the questions from your purpose. Minimal detail required - just text and arrows. A well
designed flowchart will solidify the “whole picture” with respect to your experimental
reasoning. You need to create this electronically using programs like PowerPoint, word, etc.
, Lab 1
PART A PART B
Practice pippetting techniques Calculate original stock sample of DNA
Practice pipetting various volumes of water with different micropippeter sizes
Acquire a bucket of ice
Confirm correct dispension of water withCalculate amount (L) of nuclear free-water needed to resuspend lypholized primers to a final conce
weigh boat
demonstrate accuracy with pippette to mentor Add calculated amount of nuclease free-water
into test tube
Vortex test tubes for 1-2 mins
Prepare 1/10 dilution of each stock primer, appropriately labelling them forward and reverse pr
Add PCR components to tubes 1 and 2 according to the checklist
Conduct proper disposal protocols of reagents
Wash your hands
Marking Scheme (for lab mentor use only) – for the purpose and experimental overview section, please provide a mark out
of 4, whereby:
0-0.5 0.5-1 1-1.5 1.5-2
Purpose - Is concise, uses proper grammar/scientific
Unacceptable Sufficient Accomplished Exemplary
terminology, and appropriately describes the day’s lab. 🗆 🗆 🗆 🗆
Experimental overview – is visually appealing, easy to follow 0-0.5 0.5-1 1-1.5 1.5-2
and understand, contains appropriate content and highlights Unacceptable Sufficient Accomplished Exemplary
pertinent safety features. Shows a clear understanding of 🗆 🗆 🗆 🗆
the experimental procedure.
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