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DNA Sequencing and Repair Mechanisms Review Questions and Answers Graded A+

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DNA Sequencing and Repair Mechanisms Review Questions and Answers Graded A+ Next-generation pyrosequencing - Answers A method that releases a flash of light when luciferase binds to luciferin. Spontaneous deaminination of adenine - Answers Leads to the formation of hypoxanthine. Spontaneous deam...

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  • October 21, 2024
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  • 2024/2025
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  • DNA Sequencing and Repair Mechanisms
  • DNA Sequencing and Repair Mechanisms
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DNA Sequencing and Repair Mechanisms Review Questions and Answers Graded A+

Next-generation pyrosequencing - Answers A method that releases a flash of light when luciferase binds
to luciferin.

Spontaneous deaminination of adenine - Answers Leads to the formation of hypoxanthine.

Spontaneous deaminination of cytosine - Answers Leads to the formation of uracil.

Spontaneous deaminination of 5-methylcytosine - Answers Leads to the formation of thymine.

Spontaneous deaminination of guanine - Answers Leads to the formation of xanthine.

Spontaneous deaminination of uracil - Answers Leads to the formation of thymine.

Homologous recombination - Answers Occurs between homologous sequences in sister chromatids.

Dideoxyribonucleotides - Answers Terminate polynucleotide chain extension because they lack a 3' OH
group.

Missense mutation - Answers Can lead to a change in amino acid sequence.

Nonsense mutation - Answers Can lead to the introduction of a premature stop codon in the
polypeptide chain.

Silent mutation - Answers Can lead to no change in amino acid sequence.

O6 Methyl guanine - Answers Is repaired by O6 methylguanine-DNA methyltransferase.

Restriction enzymes - Answers Bind to specific DNA sequences.

Nucleotide excision repair - Answers Involves the cleavage of damaged DNA.

Exonuclease - Answers Removes nucleotides from one end of the DNA and does so in one direction.

Endonuclease - Answers Cleaves one strand of DNA at internal sites.

Chain terminators - Answers Are used in Sanger DNA sequencing.

DNA primers in PCR - Answers Provide the DNA polymerase with a free 3' OH group for chain extension.

Next-generation reversible terminator sequencing - Answers Uses light to identify the last nucleotide
added to the growing DNA chain.

Human genome - Answers Contains 1.5 percent of protein encoding sequences.

Protein coding sequences - Answers Encode proteins of unknown function in 30 percent of the human
genome.

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