Exam (elaborations)
JCCC BIO 231 Lab Exam 2: Questions With Solutions
- Course
- Institution
JCCC BIO 231 Lab Exam 2: Questions With Solutions
[Show more]
Content preview
JCCC BIO 231 Lab Exam 2: Questions With SolutionsBacterial Smear Preparation Right Ans - 1. Place a small drop of water on a
slide
2. with the inoculating loop, aseptically add bacterial culture to the drop of
water
3. mix the bacteria with the water and spread it out
4. allow suspension to air dry
5. heat-fix the suspension (smear) by quickly passing the slide through a flame
three times.
Gram Stain Procedure Right Ans - 1. Prepare bacterial smear
2. Add Crystal violet for 1 minute
3. Rinse slide w/water then add mordant (iodine) for 1 minute
(something that helps fix dye on or in a cell but does not itself contribute
color)
4. rinse slide w/water
5. decolorize w/ Gram Alcohol by dripping down the slide until color flushes
out, rinse with water
6. Counterstain w/ Safranin for 2-3 minutes then rinse w/ water and blot dry
Acid Fast Stain Right Ans - 1. apply primary stain- carbolfuchsin
4- 5 min (rinse w/ h2o)
2. decolorize with acid-alcohol (dripping it) until color runs out then rinse w/
water
-non acid-fast bacteria decolorize,
acid-fast remain pink
3. apply counterstain- methylene blue (1 minute)
rinse w/ h20
-acid-fast are pink, non acid-fast are blue
4. blot dry
* Use Mycobacterium as positive control
Endospore Stain Right Ans - 1. Prepare bacterial smear * Bacillus +
2. Use Malachite green to flood slide and add paper towel- ensure its saturated
3. Use heat as a mordant. Flame the slide until steam appears (4-5 passes)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zendaya. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
72042 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now