Exam (elaborations)
BIOCHEM Lab Final SHSU Exam Questions and Complete Solutions Graded A+
- Course
- Institution
BIOCHEM Lab Final SHSU Exam Questions and Complete Solutions Graded A+
[Show more]
Content preview
BIOCHEM Lab FinalSHSU Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
,Micropippette - Answer: A pipette is a laboratory tool commonly used in chemistry, biology and
medicine to transport a measured volume of liquid, often as a media dispenser.
Resting-micropipette - Answer: position for when not in use or with liquid drawn
First stop on Micropipette - Answer: Load the sample
Draws indicated volume into the pipette tip
Second stop on Micropipette - Answer: Deliver the sample, completely discharging all of the liquid
Bronsted-Lowry acid - Answer: proton donor (H+)
Bronsted-Lowry base - Answer: proton acceptor
Lewis base - Answer: an atom, ion, or molecule that donates an electron pair to form a covalent bond
Lewis acid - Answer: an atom, ion, or molecule that accepts an electron pair to form a covalent bond
Henderson-Hasselbalch equation - Answer: pH = pKa + log [A-]/[HA]
conjugate base/acid
Find hydronium concentration - Answer: 10e-ph
Find pH - Answer: -log[H+]
Find pKa given Ka - Answer: -logKa
, Find Ka given pKa - Answer: 10e-pka
find pOH given [OH-] - Answer: -log[OH-]
Find [OH-] from pOH - Answer: 10e-pOH
When should you not use the henderson-hasselbach equation ?
When should you use the equation . - Answer: Henderson-Hasselbach should NOT be used for STRONG
acids.
It SHOULD be used for weak acids/base (buffer solutions)
Buffer - Answer: A solution that minimizes changes in pH when extraneous acids or bases are added to
the solution.
Tris buffer (tris hydroxymethyl aminomethane)
What is this used for in Biochemistry Lab?
What two form is it available in ? - Answer: Tris Buffer used for DNA preparations, Protein Experiments
-Exists as Tris Free-base and Tris-HCL
Tris is a weak acid with a pka of 8.075.
What does this information infer about Tris and how can we use it. - Answer: We can infer that Tris
buffer range is between 6-8.
This is ideal buffer range for biological application, with physiological pH being around 7.4
How should you prepare tris buffer ? - Answer: Dissolving mass free tris in water and adjust to desired
pH using HCL
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller YourExamplug. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75632 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now
