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HLA CHT PRACTICE EXAM QUESTIONS
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HLA CHT PRACTICE EXAM QUESTIONS
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HLA CHT PRACTICE EXAM QUESTIONSBlood specimens known to carry pathogens are packaged the same as routine
specimens..... true or false - Answers -True
When performing a blood draw, what is not part of the routine procedure?
Change gloves in between patients
Informing patient of HIV status
Wear a lab coat or gown - Answers -Informing patient of HIV status
What is a fire assembly area? - Answers -A safe place to meet during a fire, that is
predetermined by employer
Reagent x arrives in the lab, What action is the best action to do? - Answers -
Periodically QC the reagent and develop a QA analysis of its performance to derive an
appropriate expiration date
Which of the folllowing samples would be least preferred for CDC/PRA testing?
A Plain red top tube
B Serum separator tube
C Frozen serum
D ACD yellow top tube - Answers -D
Thrombin is used for what? - Answers -Clotting anti-coagulated plasma
What treatment causes the chelation of magnesium and calcium? - Answers -EDTA
What is the purpose of pronasing cells for a flow crossmatch? - Answers -To remove
CD20 from the cell surface
What does C1V1=C2V2 mean? - Answers -C1=Concentration of the stock
V1=volume to be removed from the concentrated stock solution
C2=Final concentration of the diluted solution
V2=Final volume of the diluted solution
Dead cells stained with AO/EB appear orange because
a. Ethidium bromide prevents AO binding
b. AO leaks out of the dead cell, leaving only ethidium bromide
c. Ethidium bromide fluoresces much more strongly than AO
d. AO binds only RNA, which washes away when the cell is dead
e. Dead cells are actually orange. - Answers -C
Ficoll separates lymphocytes based on what? - Answers -Density
What effect do heterophile antibodies in the complement have on the AHG-CDC assay?
, A) CDC assay complement does not contain appreciable amounts of heterophile
antibodies
B) Enhances the effectiveness of the complement
C) many false negative reactions
D) Decreased CYNAP effect - Answers -C
What is the usual source of complement used in CDC crossmatch assay? - Answers -
Rabbit
Which of the following is true about storing flourescent reagents including monoclonal
antibodies and micro array beads?
A) Ensure reagent is kept frozen as much as possible, so replace vial in freezer
between runs
B) Ensure reagent is kept in the dark to avoid photobleaching, so use light blocking vials
and store them in a closed box
C) Ensure reagent is not frozen, so leave vials at 4c or on the beach
D) Ensure reagent receives sufficient light to activate fluorescence, so leave vial on the
counter with the lights for at least 1 hour - Answers -B
The usual final concentration of DMSO for freezing cells? - Answers -10 percent
A short DNA oligonucleotide used for PCR-SSP amplification is what?
A) Probe
B) Amplicon
C) Polymerase
D) Primer
E) Okazaki fragment - Answers -Primer
Which of the listed probes with have the highest TM?
A) AACTAGTTA
B) CTATGGATCGTTGGCTACTCT
C) GTAGATTATATTACTCTAGCA
D) AACTAGTTC
E) ATATATATAT - Answers -C...Look for the most G/Cs
Primers and probes bind to their DNA targets through a process known as
a) High stringency
b) Hydrogen bonding
c) Covalent bonding
d) Primer dimerism
e) Low Stringency - Answers -B Hydrogen Bonding
Which is true of the 3' and/or 5' ends of an oligonucleotide primer?
a) The 3' and 5' ends always have purines
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