Exam (elaborations)
ACS Biochem Final Test Questions with correct Answers
- Course
- Institution
ACS Biochem Final Test
[Show more]
Content preview
ACS Biochem Final Testnet reaction for glycolysis - answer2x Pyruvate + 2x ATP + 2x NADH per glucose
molecule
what step produces 2x NADH in glycolysis? - answerG3P -> 1,3 BPG
What enzyme/pathway does ATP inhibit? – answer A high level of ATP inhibits the
enzyme PFK-1 by decreasing its affinity for fructose 6-phosphate. AMP diminishes and
citrate enhances the inhibitory effect of ATP.
The NADPH produced by HMP is used for - answer reductive biosynthesis (anabolic)
how do competitive inhibitors affect Km and Vmax? - answer increases Km, doesn't
change Vmax
how do noncompetitive inhibitors affect Km and Vmax? – answer Km doesn't change,
Vmax lowers
SDS-PAGE - answer Type of chromatography used to separate proteins based on
mass. SDS binds to the proteins, giving them a negative charge cancelling the effect of
charges from the individual amino acids. Lighter proteins travel faster than heavier
ones.
PCR - answerpolymerase chain reaction- DNA is copied multiple times to produce many
copies of the original molecules helpful when there's only a small DNA sample
Arginine (pka) - answerPositively charged R groups (pKa=12.5)
Aspartic Acid (pka) - answerNegatively charged R groups (pKa=3.9)
Cysteine (pka) - answerPolar, uncharged R groups (pKa=8.3)
Glutamic Acid (pka) - answerNegatively charged R groups (pKa=4.3)
Histidine (pka) - answerPositively charged R groups (pKa=6.0)
Lysine (pka) - answerPositively charged R groups (pKa=10.5)
Tyrosine (pka) - answerPositively charged R groups (pKa=10.1)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller julianah420. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
78121 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now