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BGM3039 - L14 Exam Latest Update
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BGM3039 - L14 Exam LatestUpdate
what are some problems with studying redox proteins at electrode surfaces - Answer
-redox-active centre often shielded by protein so electron transfer slow/not possible
-slow protein diffusion
-stability of proteins limited outside natural environment so may degrade = lose
co-factor
-proteins adsorption on bare electrodes = denaturation
what are the 2 classes of redox enzymes - Answer extrinsic
intrinsic
what is an example of an extrinsic enxyme - Answer cytochrome c peroxidase
how do extrinsic redox enzymes work - Answer -electron transfer in vicinity of sensor
-own protein acts as a cofactor
what is the mechanism of cytochrome c peroxidase - Answer -convert ROS to water
-ferrous cc acts as electron receiver to reduce H2O2 to water
CCP+H2O2+Fe2+2H+ -> CCP+2H2O+2H+
how do intrinsic redox enzymes work - Answer -electron transfer within enzyme =
difficult to achieve
-co-substrate required
why are intrinsic enzymes modified - Answer -difficult transfer: buried active site,
denatures at surface
-modified = allows closer orientation
what is an example of an intrinsic redox enzyme - Answer glucose oxidase
how do the 3 generations of biosensors differ - Answer 1 = no direct connection with
enzyme and electrode
2 = use of a mediator to link active site to electrode
3 = direct electron transfer between enzyme and electrode
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