Exam (elaborations)
MIBO 3510 L EXAM 1 UGA
- Course
- Institution
MIBO 3510 L EXAM 1 UGA
[Show more]
Content preview
MIBO 3510 L EXAM 1 UGAbiosafety level 1 - Answers -Well-characterized agents which do not cause disease in
healthy humans
-ex: e. coli
What causes bacteria to have different morphologies? - Answers --Growth rate (size)
-Motility (rate of spread)
-Pigment production
-Cell wall composition/polysaccharide production
-Media composition
isolation of pure cultures - Answers -separating cells physically (streak plates) or by
dilution (spread and pour plates)
streak plate technique - Answers -A small amount of bacteria is spread over the surface
of an agar plate
- not a quantitative method
(spreading tool must be sterilized between streaks)
Spread and pour plates - Answers -allow enermeration of cells as well as isolation
-> quantitative
Cultures are serially diluted (10-fold sets) before cells are transferred to the agar
-Spread: Diluted culture pipetted on top of agar and spread over surface with a glass
rod
-Pour: Diluted culture is pipetted into warm agar and stirred, then poured into a sterile
dish
What are the 2 energy sources for metabolic classification? - Answers -1. light (photo)
2. chemicals (chemo)
photo - Answers -energy source is light
chemo - Answers -energy source is chemicals
What are the 2 electron sources for metabolic classification? - Answers -1. organic
(organo)
2. inorganic (litho)
organo - Answers -electron source: organic
litho - Answers -electron source is inorganic (FeS. NH3, H2, etc.)
, What are the 2 carbon sources for metabolic classification? - Answers -1. organic
(hetero)
2. CO2 (auto)
Heterotroph - Answers -carbon source is organic (sugars, etc)
autotroph - Answers -carbon source is carbon dioxide (which they fix themselves to get
carbon)
What primary elements are carbohydrates made of? - Answers -CHO
What primary elements are lipids made of? - Answers -CHOPN
What primary elements are nucleic acids made of? - Answers -CHOPN
What primary elements are proteins made of? - Answers -CHONS
What does bacteriological media contain? - Answers --Major nutrients: C, N, P, S +
energy source
-Salts for osmotic balance
-May include: trace elements, vitamins
-semi-solid and solid mediums also contain either gelatin or agar as a solidifying agent
What is defined media? - Answers -aka synthetic media
All components (and their exact concentrations) are known
common ingredients:
-Carbon - Often glucose, other sugars
-Nitrogen - Ammonium (NH4+) or nitrate (NO3-)
-Phosphorus - Often added as buffer (K2HPO4 and KH2PO4
-Sulfate - SO4
What is complex media? - Answers -Biological mixtures/digests added, exact
composition unknown
-best for growing a variety of cultural organisms
common ingredients:
-Yeast extract
-Tryptone
-Peptone
What is a fastidious organism? - Answers -requires complex nutritional requirements
from the environment
-will only grow when specific nutrients are available
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller GEEKA. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
80364 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now