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GCD EXAM 3 WITH COMPLETE QUESTIONS AND ANSWERS
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GCD EXAM 3 WITH COMPLETE QUESTIONSAND ANSWERS
Which of the following may be used as a vector in a gene-cloning experiment?
a) mRNA
b) Plasmid
c) Virus
d) c & d - ANSWER d
The restriction enzymes used in gene-cloning experiments ___________, which
generates sticky ends that can _________.
a. cut the DNA, enter bacterial cells
b. cut the DNA, hydrogen bond with complementary sticky ends
c. methylate DNA, enter bacterial cells
d. methylate DNA, hydrogen bond with complementary sticky ends - ANSWER
b
Which of the following is the proper order of steps in a gene-cloning experiment
involving vectors?
1. Add DNA ligase
2. Incubate the chromosomal DNA and the vector DNA with a restriction enzyme.
3. Introduce the DNA into living cells
4. Mix the chromosomal DNA
a. 1,2,3,4
b. 2,3,1,4
c. 2,4,1,3
d. 1,2,4,3 - ANSWER c
the function of reverse transcriptase is to:
, a. copy RNA into DNA
b. copy DNA into RNA
c. translate RNA into proteins
d. translate DNA into protein - ANSWER a
In one PCR cycle, the correct order of steps is:
a. primer annealing, primer extension, denaturation
b. primer annealing, denaturation, primer extension
c. denaturation, primer annealing, primer extension
d. denaturation, primer extension, primer annealing - ANSWER c
In reverse transcriptase PCR, the starting biological material is
a. chromosomal DNA
b. mRNA
c. proteins
d. all of the above - ANSWER b
During real time PCR, the synthesis of PCR products is analyzed
a. at the very end of the reaction by gel electrophoresis
b. at the very end of the reaction by fluorescence that is emitted within the
thermocycler
c. during the PCR cycles by gel electrophoresis
d. during the PCR cycles by fluorescence that is emitted within the thermocycler
- ANSWER d
When a dideoxyribonucleotide is incorporated into a growing strand,
a. the strand elongates faster
b. the strand cannot elongate
c. the stand becomes more susceptible to DNase I cleavage.
d. none of the above happens - ANSWER b
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