Exam (elaborations)
UCF Biomed Exit Exam Solved 100% Correct!!
- Course
- Institution
UCF Biomed Exit Exam Solved 100% Correct!!
[Show more]
Content preview
UCF Biomed Exit Exam Solved 100% Correct!!Griffith's experiment Answer- Showed that bacteria can acquire DNA through transformation
Two strains of Pneumococcus; DNA from pathogenic strain taken up by non-
pathogenic strain, which then became pathogenic
Avery's experiment Answer- DNA, not proteins, caused bacterial transformation and is therefore the carrier of genetic information
Meselson's experiment Answer- Showed that DNA replication is semi-conservative
McClintock's experiment Answer- Discovered transposons
Hershey-Chase experiment Answer- Showed that only the DNA, no proteins, from bacteriophage enters a bacteria.
This demonstrates that genetic information is carried in DNA.
Watson and Crick Answer- Generated the double-stranded helix model of DNA
Sliding clamp Answer- Part of the DNA Pol. 3 holoenzyme
Prevents the polymerase from dissociating from the template DNA strand
DNA Helicase Answer- Unwinds DNA, separating the two strands
Topoisomerase Answer- Alters the supercoiled form of DNA
Telomerase Answer- Adds a species-dependent telomere repeat sequence to the 3' end of telomeres without the need for a DNA template
Order in which enzymes participate in DNA replication Answer- Topoisomerase
Helicase
Single-stranded binding proteins
Primase DNA polymerase 3 holoenzyme
DNA polymerase 1
- Removes RNA primers and fills in gaps between Okazaki fragments
Ligase
Activator proteins Answer- A DNA-binding proteins that recruit transcriptional machinery
Enhancers Answer- The region of DNA that activators bind to
Silencers Answer- DNA sequences that repressor proteins bind to in order to decrease transcription
Riboswitches Answer- Regulatory RNA elements within the mRNA that act as direct sensors of, small molecules, tRNA, temperature, pH, metals, altering the rate of production of the product of that mRNA in prokaryotes
Processing of eukaryotic mRNA Answer- Transcription
Capping
- Added almost immediately after transcription begins
Polyadenylation
RNA splicing
mRNA export from the nucleus via mRNA transporter
Parallel vs. antiparallel β sheets Answer- In parallel, the H bonds are at an angle, whereas in anti-parallel, they are straight.
Origins of multidomain proteins Answer- Gene duplications (retrotransposons, unequal crossing over, DNA polymerase slippage, nondisjunction, genome duplication) followed by divergence
Base pair recognition in major vs minor grooves Answer- Major groove:
-There is enough room to recognize arrangement of H bond donors and acceptors
--- Can tell discriminate between CG, GC, AT, and TA
Minor groove:
- Can only discriminate between CG/GC and AT/TA but won't be able to tell whether it's CG or GC
Leucine zipper domain Answer- Family of DNA binding proteins where two subunits come together to form a dimer through use of coiled coil region.
- The DNA goes between the two subunits
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller EvaTee. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
81298 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now