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Electrophoresisi, Chromatography, Molecular – Questions And Answers
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Electrophoresisi, Chromatography, Molecular –Questions And Answers
Which molecular method uses fluorescent probes to evaluate genes and/or
DNA sequences on chromosomes? ✔️Ans - Fluorescence In Situ
Hybridization (FISH)
Some real-time PCR instruments have the ability to perform melting curve
analysis. What is the melting temperature (Tm) for primers? ✔️Ans - The
temperature at which DNA denatures into two strands and is dependent on
the nucleotide composition of the molecule.
Which protein is represented by the band labeled by the number 4 on the SPE
scan? ✔️Ans - Albumin
Which nitrogen base would bind with a guanine nucleotide in forming double-
stranded DNA? ✔️Ans - Cytosine
All of the following test principles are matched with the most appropriate
response, EXCEPT ✔️Ans - TMA - Target amplification using reverse
transcriptase. Thermal cycling is needed.
All of the following would are considered molecular testing techniques used in
the clinical laboratory to identify unique nucleic acid sequences, EXCEPT
✔️Ans - Immunoassays
This serum protein electrophoresis (SPE) scan most likely represents which of
the following disease states: ✔️Ans - Multiple myeloma
You are analyzing the results of a qPCR for Cytomegalovirus. Several of the
samples have a failed internal control. Which is not a reason why the internal
control might have failed? ✔️Ans - The samples have low viral loads
The following steps were used in a procedure to identify M. kansasii:
1 - Extraction2 - Denaturization3 - Hybridization4 - Hydrolysis of excess
reagent5 - Detection of acridinium ester
Which of the following methods has been described? ✔️Ans - Nucleic acid
probes
, What are the four nitrogenous bases that are found in RNA? ✔️Ans -
Adenine, guanine, cytosine, and uracil
In clinical genetics, the major consequence of chromosomal deletion: ✔️Ans
- Is the loss of specific genes that are localized to the deleted chromosomal
segment.
All of the following are factors that will affect migration in gel electrophoresis,
EXCEPT? ✔️Ans - Amount of light present
In a PCR amplification method, which of the following is amplified? ✔️Ans -
Target
Which of the following probe types is used to detect a translocation? ✔️Ans -
Break-apart probes
Which of the following chromatography systems is characterized by a
stationary phase of silica gel on a piece of glass and a mobile phase of liquid?
✔️Ans - Thin-layer
What is a disadvantage to molecular testing methods, such as nucleic acid
amplification? ✔️Ans - Specialized detection equipment
Proteins in a buffer that sets the pH at 8.6 will become ___________ charged and
migrate to the ____________. ✔️Ans - Negatively; Anode
The following diagram demonstrates two Serum Protein Electrophoresis
(SPEP) patterns from different patients. The pattern on the left is from a
normal, healthy patient. The pattern on the right is from a patient with a
particular disorder.
What abnormality is suggested by the pattern on the right? ✔️Ans - X-
linked agammaglobulinemia
Serum proteins can be separated by cellulose acetate electrophoresis into how
many basic fractions: ✔️Ans - 5 fractions
What causes polyacrylamide gels to have greater resolution of proteins than
agarose gels? ✔️Ans - Varying pore size in each polyacrylamide layer
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