LS23L Final Study Guide Exam Questions And Answers 2023/2024 | Verified Answers
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LS23L Final Study Guide Exam Questions And Answers 2023/2024 | Verified Answers
LS23L Final Study Guide Exam Questions And Answers 2023/2024 | Verified Answers
LS23L Final Study Guide Exam Questions And Answers 2023/2024 | Verified Answers True or False: According to the Research Information Sheet, you are required to participate in the Memory Interference Test (MIT) in order to s atisfactory complete this course. Correct Answer: False What does MIT stand for? Correct Answer: Memory Interference Test According to the Research Information Sheet, possible risks you assume while participating in the Memory Interference Test (MIT) inc lude: Correct Answer: Fatigue/tiredness True or False: According to the Research Information Sheet, you will be paid for participating in this study. Correct Answer: False Which of the following is not a type of MIT that may be taken? Correct Answer: Physical Choose the false statement concerning information from the Research Information Sheet Correct Answer: The database will provide you with demographic information about all subgroups as long as the contain at least two people. How should you perform the MIT? Correct Answer: Using your index finger only What statistical test will be used for the MIT lab? Correct Answer: Student t -test According to the Research Informati on Sheet, what is a benefit to subject and/or society from participating in this study? Correct Answer: You will help develop better ways to evaluate cognition in people What is the statistical test that will be used in this week's lab? Correct Answer: St udent's t-test A p-value of 0.04 obtained from a two -tailed t -test means that there is a ____ % chance that the obtained results are due to _______. Given the conventional significance level, you would accept that the _________ hypothesis is correct. Corr ect Answer: 4, chance, alternative Which of the following is not true regarding the p -value? Correct Answer: A p -value less than 0.05 means you should accept your null hypothesis. What two factors does the p -value depend on? Correct Answer: standard devi ation and mean In statistics the t -test takes into account the difference between: Correct Answer: the means and the standard error of the two samples. According to the lab safety sheet, during the epidemiology lab activity, you must wear: Correct Answer : standard lab attire, safety goggles, lab coat, and a glove on your non -
dominant hand What is the minimum volume you can safely and accurately pipet with a p1000 pipetter? Correct Answer: 100 ul Which of the following pieces of information does not need to be included when you label you plate for the epidemiology exercise? Correct Answer: Your age How many rounds of handshaking will the epidemiology exercise have? Correct Answer: 4 Which of the following factors is mentioned as contributing to the spread of disease? a. Natural environment of a region b. Cultural and social practices c. genetic background of a population d. Infrastructure present in a nation e. All of the above Correc t Answer: e. All of the above Which of the following statements about pipetting is false? a. The second stop on the pipetter should never be used under any circumstances. b. It is important that you always hold the pipetter vertically if the tip contains liquid. c. You should never handle liquid with a pipetter that has not been fitted with a tip. d. When aspirating sample, you should depress the pushbutton to the first positive stop only. e. When expelling sample, you should depress the pushbutton to the second stop. Correct Answer: A. The second stop on the pipetter should never be used under any circumstances. Assume you are patient zero (i.e. infected with bacteria) in the epidemiology lab and there were two rounds of handshakes. If you shook hands wi th two uninfected persons the first round, and each of them shook hands with two more uninfected persons the second round, how many total people would be infected including yourself? You did not shake hands with anyone else in the second round and there we re no double -infections. Correct Answer: 7 Why is it most important to learn to use pipettes correctly? Correct Answer: Pipettes are important and omnipresent piece of equipment in biology laboratories In a P1000 pipetter, the numbers read 094 from the t op down. How many micrometers is this? Correct Answer: 940 A volume of liquid measuring __ is most accurately measured and dispensed using a __ pipetter. Correct Answer: 0.12 ml, p200 What type of blank should be used for the b -galactosidase enzyme activ ity assay? Correct Answer: "b -gal blank" made by your group What is the purpose of ONPG in this lab? Correct Answer: substrate that gives a yellow color when cleaved What is the purpose of Luria broth in this lab (b -galactosidase assay) Correct Answer: provides nutrient for bacteria growth According to the Lab Safety Sheet provided for this week, when working with E. coli cells you must wear: (check all that apply) Correct Answer: Safety goggles, standard lab attire, lab coat, and gloves. True or False: In the b -galactosidase assay lab, used microcentrifuge tubes and pipette tips contaminated with E. coli should be disposed of in the regular trash. Correct Answer: False In a medium where lactose is the only carbon source, transcription of the lac operon is _______, because lac repressor is ________ to the ________. Correct Answer: unblocked, not bound, operator In the B -galactosidase assay, it is important to measure the density of E. coli cells because: Correct Answer: E. coli densities v ary among cultures and with time, which will affect estimates of b -galactosidase production rates A class is studying b -galactosidase activity in a bacterial culture. After a student adds ONPG to their sample and incubates for 10 minutes, the student then adds 10 uL PopCulture, mixes the samples, and allows the samples to sit briefly before taking an enzymatic assay reading using a spectrophotometry. To the student's surprise, their enzymatic assay reading was significantly lower than the rest of the class 's. What is the best explanation for this? Correct Answer: The student added ONPG to the bacterial cells before lysing the cells. This does not allow the b -galactosidase to come into contact with ONPG during the incubation period. In the "biochemical assa y of b -galactosidase activity" what is o -nitrophenyl -b-D-
galactosidase (ONPG) used for? Correct Answer: When ONPG is cleaved by b -
galactosidase, we were able to assay b -galactosidase activity. What will you measure in this experiment as an indicator of me tabolism (Metabolism in Goldfish Lab) Correct Answer: Oxygen concentration True or False: Students are going to design an experiment flowchart with detailed steps of experimental procedures. (Metabolism in Goldfish Lab) Correct Answer: True
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