Summary
Summary BIO 171_Lab_4_Notebook PL #4 Introduction Growth Media
- Course
- BIO 171
- Institution
- Portage Learning
BIO 171_Lab_4_Notebook PL #4 Introduction Growth Media
[Show more]
Content preview
Lab 4 NotebookBack to Home Page
Title: PL #4 Introduction Growth Media
Objective: T o learn and understand different types of growth media in order to isolate and identify unknown samples
Procedure: 4- Phase Dilution Streaking: Clonal Isolation
1.Using sterile loop spread culture in area #1 ONCE
2.Using a NEW sterile loop drag through the end of area #1 ONCE
3.Using a NEW sterile loop drag through the end of area #2 ONCE
4.Using a NEW sterile loop drag through the end of area #3 ONCE
*Use a back and forth pattern to dilute the culture in each zone.
*Invert plate and incubate overnight at 37C
Non - selective agar
Quadrant Growth: Rapid test for multiple isolates
1.Using sterile loop spread unknown culture A in area #1
2.Using a new sterile loop spread unknown culture B in area #2
3.Using a NEW sterile loop spread unknown culture C in area #3
4.Using a NEW sterile loop spread unknown culture D in area #4
*Use a back and forth pattern to dilute the culture in each zone.
* Invert plate and incubate overnight at 37C
Notes: This study source was downloaded by 100000834490709 from CourseHero.com on 11-03-2021 07:51:24 GMT -05:00
https://www.coursehero.com/file/78838942/bio-171-Lab-4-Notebookdocx/This study resource was
shared via CourseHero.com
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller irvingAplus. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
80364 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now